活體成像熒光染料是一類用于生物醫學研究中對組織、細胞和生物分子等進行標記和定位的染料，它們常用于活體成像實驗[1][2]。 百靈威提供的此系列染料具有以下優點：
- 品種齊全，提供多種脂溶性和水溶性熒光染料
- 光穩定性好，價格具有競爭力
- 適用性廣，儀器兼容性高
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產品編號
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產品名稱
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CAS
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規格
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2136044
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Cyanine3 carboxylic acid, 95%
Cy3 羧酸, 95%
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1144107-76-5
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5 mg
25 mg
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2136047
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Cyanine5.5 carboxylic acid, 99%
Cy5.5 羧酸, 99%
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1144107-80-1
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5 mg
25 mg
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1871751
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Sulfo-cyanine3 carboxylic acid, 95%
磺基-Cy3羧酸, 95%
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146368-13-0 |
5 mg
25 mg
50 mg
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1871752
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Sulfo-cyanine5 carboxylic acid, 95%
磺基-Cy5羧酸, 95%
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146368-11-8 |
5 mg
25 mg
50 mg
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1871754
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Sulfo-cyanine7 carboxylic acid, 90%
磺基-Cy7羧酸, 90%
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943298-08-6
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5 mg
25 mg
50 mg
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1871749
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Sulfo-cyanine3 NHS ester, 90%
磺基-Cy3-N-羥基琥珀酰胺酯, 90%
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146368-16-3
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5 mg
25 mg
50 mg
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1871750
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Sulfo-cyanine5 NHS ester, 90%
磺基-Cy5-N-羥基琥珀酰胺酯, 90%
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146368-14-1
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5 mg
25 mg
50 mg
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1871753
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Sulfo-cyanine7 NHS ester, 90%
磺基-Cy7-N-羥基琥珀酰胺酯, 90%
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477908-53-5
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5 mg
25 mg
50 mg
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圖 1: Confocal fluorescence images for intracellular localization of PNCy3Cy5 in macrophages. Cells were treated with 10 μM PNCy3Cy5 for 0.5 h and stained (0.5 h) with 200 nM Mito-Tracker Green, 200 nM Lyso-Tracker DND-26, or 5 μg/mL Hoechst 33342. Red channel at 640−680 nm. Green channel at 500−540 nm for Mito-Tracker Green and Lyso-Tracker Green, 440−480 nm for Hoechst 33342(341154)[1].
References
[1] Jia X, Chen Q, Yang Y, et al. FRET-Based Mito-Specific Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5[J]. Journal of the American Chemical Society, 2016, 138(34): 10778-10781.
[2] Spaide R F, Jr K J, Cooney M J. Retinal vascular layers imaged by fluorescein angiography and optical coherence tomography angiography.[J]. Jama Ophthalmology, 2015, 133(1):45.
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